Tobacco plants over-expressing the sweet orange tau glutathione transferases (CsGSTUs) acquire tolerance to the diphenyl ether herbicide fluorodifen and to salt and drought stresses.
Identifieur interne : 000404 ( Main/Exploration ); précédent : 000403; suivant : 000405Tobacco plants over-expressing the sweet orange tau glutathione transferases (CsGSTUs) acquire tolerance to the diphenyl ether herbicide fluorodifen and to salt and drought stresses.
Auteurs : Luca Lo Cicero [Italie] ; Panagiotis Madesis [Grèce] ; Athanasios Tsaftaris [Grèce] ; Angela Roberta Lo Piero [Italie]Source :
- Phytochemistry [ 1873-3700 ] ; 2015.
Descripteurs français
- Wicri :
- geographic : Grèce.
English descriptors
- KwdEn :
- Citrus sinensis (enzymology), Citrus sinensis (genetics), DNA (genetics), Droughts, Glutathione Transferase (metabolism), Glutathione Transferase (pharmacokinetics), Greece, Halogenated Diphenyl Ethers (pharmacokinetics), Halogenated Diphenyl Ethers (pharmacology), Herbicide Resistance, Plants, Genetically Modified (chemistry), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (genetics), Salinity, Salt-Tolerance (genetics), Salt-Tolerance (physiology), Stress, Physiological (genetics), Tobacco (chemistry), Tobacco (enzymology), Tobacco (genetics).
- MESH :
- chemical , genetics : DNA.
- chemical , pharmacokinetics : Halogenated Diphenyl Ethers.
- chemical , pharmacology : Halogenated Diphenyl Ethers.
- geographic : Greece.
- chemistry : Plants, Genetically Modified, Tobacco.
- enzymology : Citrus sinensis, Plants, Genetically Modified, Tobacco.
- genetics : Citrus sinensis, Plants, Genetically Modified, Salt-Tolerance, Stress, Physiological, Tobacco.
- chemical , metabolism : Glutathione Transferase.
- chemical , pharmacokinetics : Glutathione Transferase.
- physiology : Salt-Tolerance.
- Droughts, Herbicide Resistance, Salinity.
Abstract
The glutathione transferases (GSTs) are members of a superfamily of enzymes with pivotal role in the detoxification of both xenobiotic and endogenous compounds. In this work, the generation and characterization of transgenic tobacco plants over-expressing tau glutathione transferases from Citrus sinensis (CsGSTU1 and CsGSTU2) and several cross-mutate forms of these genes are reported. Putative transformed plants were verified for the presence of the transgenes and the relative quantification of transgene copy number was evaluated by Taqman real time PCR. The analysis of gene expression revealed that transformed plants exhibit high levels of CsGSTU transcription suggesting that the insertion of the transgenes occurred in transcriptional active regions of the tobacco genome. In planta studies demonstrate that transformed tobacco plants gain tolerance against fluorodifen. Simultaneously, the wild type CsGSTU genes were in vitro expressed and their kinetic properties were determined using fluorodifen as substrate. The results show that CsGSTU2 follows a Michaelis-Menten hyperbolic kinetic, whereas CsGSTU1 generates a sigmoid plot typical of the regulatory enzymes, thus suggesting that when working at sub-lethal fluorodifen concentrations CsGSTU2 can counteract the herbicide injury more efficiently than the CsGSTU1. Moreover, the transgenic tobacco plant over-expressing CsGSTs exhibited both drought and salinity stress tolerance. However, as we show that CsGSTUs do not function as glutathione peroxidase in vitro, the protective effect against salt and drought stress is not due to a direct scavenging activity of the oxidative stress byproducts. The transgenic tobacco plants, which are described in the present study, can be helpful for phytoremediation of residual xenobiotics in the environment and overall the over-expression of CsGSTUs can be helpful to develop genetically modified crops with high resistance to abiotic stresses.
DOI: 10.1016/j.phytochem.2015.03.004
PubMed: 25819876
Affiliations:
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Le document en format XML
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Electronic address: rlopiero@unict.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agriculture, Food and Environment (Di3A), University of Catania, Via Santa Sofia 98, 95123 Catania</wicri:regionArea><wicri:noRegion>95123 Catania</wicri:noRegion></affiliation></author></analytic><series><title level="j">Phytochemistry</title><idno type="eISSN">1873-3700</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus sinensis (enzymology)</term><term>Citrus sinensis (genetics)</term><term>DNA (genetics)</term><term>Droughts</term><term>Glutathione Transferase (metabolism)</term><term>Glutathione Transferase (pharmacokinetics)</term><term>Greece</term><term>Halogenated Diphenyl Ethers (pharmacokinetics)</term><term>Halogenated Diphenyl Ethers (pharmacology)</term><term>Herbicide Resistance</term><term>Plants, Genetically Modified (chemistry)</term><term>Plants, Genetically Modified (enzymology)</term><term>Plants, Genetically Modified (genetics)</term><term>Salinity</term><term>Salt-Tolerance (genetics)</term><term>Salt-Tolerance (physiology)</term><term>Stress, Physiological (genetics)</term><term>Tobacco (chemistry)</term><term>Tobacco (enzymology)</term><term>Tobacco (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Halogenated Diphenyl Ethers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Halogenated Diphenyl Ethers</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Greece</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus sinensis</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>Plants, Genetically Modified</term><term>Salt-Tolerance</term><term>Stress, Physiological</term><term>Tobacco</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Salt-Tolerance</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Droughts</term><term>Herbicide Resistance</term><term>Salinity</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Grèce</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The glutathione transferases (GSTs) are members of a superfamily of enzymes with pivotal role in the detoxification of both xenobiotic and endogenous compounds. In this work, the generation and characterization of transgenic tobacco plants over-expressing tau glutathione transferases from Citrus sinensis (CsGSTU1 and CsGSTU2) and several cross-mutate forms of these genes are reported. Putative transformed plants were verified for the presence of the transgenes and the relative quantification of transgene copy number was evaluated by Taqman real time PCR. The analysis of gene expression revealed that transformed plants exhibit high levels of CsGSTU transcription suggesting that the insertion of the transgenes occurred in transcriptional active regions of the tobacco genome. In planta studies demonstrate that transformed tobacco plants gain tolerance against fluorodifen. Simultaneously, the wild type CsGSTU genes were in vitro expressed and their kinetic properties were determined using fluorodifen as substrate. The results show that CsGSTU2 follows a Michaelis-Menten hyperbolic kinetic, whereas CsGSTU1 generates a sigmoid plot typical of the regulatory enzymes, thus suggesting that when working at sub-lethal fluorodifen concentrations CsGSTU2 can counteract the herbicide injury more efficiently than the CsGSTU1. Moreover, the transgenic tobacco plant over-expressing CsGSTs exhibited both drought and salinity stress tolerance. However, as we show that CsGSTUs do not function as glutathione peroxidase in vitro, the protective effect against salt and drought stress is not due to a direct scavenging activity of the oxidative stress byproducts. The transgenic tobacco plants, which are described in the present study, can be helpful for phytoremediation of residual xenobiotics in the environment and overall the over-expression of CsGSTUs can be helpful to develop genetically modified crops with high resistance to abiotic stresses.</div></front></TEI><affiliations><list><country><li>Grèce</li><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Lo Cicero, Luca" sort="Lo Cicero, Luca" uniqKey="Lo Cicero L" first="Luca" last="Lo Cicero">Luca Lo Cicero</name></noRegion><name sortKey="Lo Piero, Angela Roberta" sort="Lo Piero, Angela Roberta" uniqKey="Lo Piero A" first="Angela Roberta" last="Lo Piero">Angela Roberta Lo Piero</name></country><country name="Grèce"><noRegion><name sortKey="Madesis, Panagiotis" sort="Madesis, Panagiotis" uniqKey="Madesis P" first="Panagiotis" last="Madesis">Panagiotis Madesis</name></noRegion><name sortKey="Tsaftaris, Athanasios" sort="Tsaftaris, Athanasios" uniqKey="Tsaftaris A" first="Athanasios" last="Tsaftaris">Athanasios Tsaftaris</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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